Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Microbial Isolates from Urine Samples of Patients Presenting with Dysuria and Urinary Frequency at Agbor: Is Staphylococcus aureus a Urinary Pathogen?

T.C. Ozoko¹, G.E. Umukoro¹, I. Onyesom²* and S.I. Ovuakporaye³

¹Department of Medical Microbiology and Parasitology, ²Department of Medical Biochemistry, ³Department of Physiology, Delta State University, Abraka, Nigeria.

Received on 21 April 2008 and accepted on 02 July 2008

 

ABSTRACT

Urine samples taken by clean catch method from 210 adults presenting on account of dysuria and urinary frequency between January to June 2006 were analysed. Samples were cultured for 24 hours using MacConkey agar, chocolate agar and cysteine lactose electrolyte deficient agar. Isolates were identified using colony morphologic characteristics, gram stain characteristics, catalase test, coagulase test, indole test, oxidase test, krigler-iron agar, and germ tube test. 81 samples(38.57%) grew at least105(> 105) colony forming units per ml. The organisms isolated were Escherichia coli 36.90%,Staphylococcus aureus 25%, Klebsiella spp 19.05%, Proteus spp 15.48%, Pseudomonas aeruginosa 1.19%, Enterococcus faecalis 1.19%, Candida albicans 1.19%.Male to female ratio of the isolates is 28.4% to 71.6% (1:2.52). The present study shows that Escherichia coli is the commonest cause of urinary tract infection. This study shows a relatively high yield of Staphylococcus aureus and this suggests that this uncommon commensal of the anterior third of the urethra is a pathogen. Isolation of Candida albicans points to the increasing significance of funguria as a component of urinary tract infection. Our data show a significant difference from usual Caucasian patterns.

Keywords : Clean catch, commensal, pathogen, funguria.