Journal of Pure and Applied MicrobiologyVol. 7 No. Special Edition

Protective Effects of Total Flavonoids from Dracocephalum moldavica L. on Myocardial Ischemia /Reperfusion Injury in the Isolate Rats Heart

Yu Bacui1, Wang Ting1, Zhang Bo, Wang Zhenhua* and Zheng Qiusheng*

Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi - 832 000, China.

Received on 03 March 2013 and accepted on 14 April 2013

 

ABSTRACT

The purpose of this study was to examine the cardioprotective effects of total flavonoids from Dracocephalum moldavica L. (DML) on ischemia/reperfusion injury (I/R) using an isolated Langendorff rat heart preparation. Hearts were perfused with Krebs-Henseleit (K-H) solution containing DML (100 ug/L) for 10 min before 20-minute global ischemia, then reperfusion was begun with K-H solution for 40 min. The left ventricular developed pressure (LVDP) and maximum up/down rate of left ventricular pressure (ądp/dtmax) were recorded by physiological recorder as the myocardial function. And the level of superoxide dismutase (SOD), malondialdehyde (MDA) and GSH/GSSG ratio were analyzed to determine the oxidative stress status in myocardial tissue. Compared with I/R injury group, pre-incubation with DML (100 ug/L) significantly improved LVDP and ądp/dtmax during reperfusion. Notably, the level of MDA significantly decreased (P<0.01) in DML group compared to I/R injury group, as well as, the SOD activity (P<0.01) and total glutathione (P<0.05) increased. The results implied that the DML has obviously protective effects on myocardial reperfusion injury.

Keywords : Myocardial Ischemia/Reperfusion, SOD, MDA, GSH/GSSG.