Journal of Pure and Applied MicrobiologyVol. 7 No. 1

Experimental Study on Pigment Epithelium Derived Factor Combined with Macrophage Conditioned Medium on Rat Retinal Ganglion Cells Axonal Regeneration Influence

Jin-Yuan Wu*, Feng-Yuan Sun, Dong-Run Tang and Rui Zhang

Department of Ophthalmology, Tianjin First Center Hospital, Tianjin, China.

Received on 09 January 2013 and accepted on 28 February 2013



The study of pigment epithelium derived factor (PEDF) combined with macrophage conditioned medium (MCM) could synergetically protect retinal ganglion cells (RGCs) survival and promote axonal regeneration of RGCs purpose, by macrophages and yeast polysaccharide and preparation of culture cell slide and MCM cell of experimental method, draw a conclusion, separate application PEDF cannot protect cultured RGCs survival and axonal regeneration promotion, MCM can protect RGCs survival and promotes axonal regeneration, and PEDF can be synergistic protective RGCs survival and promotes axonal regeneration.

Keywords : Pigment epithelium derived factor, Macrophage conditioned medium, Rat, Retinal ganglion cells, Axons, Regeneration.