Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Screening of Microorganisms for Azo Dye Degradation from Dye Affected Sites of Sanganer, Rajasthan, India.

Archana sharma1*, Anamika2 and Jyoti Saxena2

1Biophysics and Single Molecule Manipulation Lab, Physics Department, Blk S12, Faculty of Science, National University of Singapore, 2 Science Drive 3, 117 542, Singapore. 2Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali - 304 022, India.

Received on 02 April 2008 and accepted on 06 May 2008



The present study deals with the screening of microorganisms for azo dye degradation. The bacteria and fungi were isolated from soil and effluent samples collected from different sites in Sanganer, Rajasthan and were screened for their degradation potential along with 14 isolates of Aspergillus (previously isolated from Banasthali University agricultural soil), on 3 commonly used azo dyes, viz., congo red, methyl orange and methyl red. Eleven bacterial isolates (5 Gram positive and 6 Gram negative) and 4 fungal isolates (2 Aspergillus sp. belonging to niger group, A. ochraceous and A. flavus) obtained from the dye affected soil and effluent samples and A. niger, A. japonicus, A. aculeatus, A. foetidus and A. purpurogenum from the agricultural soil showed dye decolourization activity. Bacterial isolates 2 and 4 showed 83-94% degradation, whereas, fungal strains A. japonicus and A. aculeatus showed 95-98% of decolourization of all the 3 dyes studied. The activity shown by the referred bacterial and fungal strains was more than Pseudomonas putida, the reference strain. The dye removal was achieved by both biodegradation as well as bisorption processes. In general, fungal isolates were more efficient dye degraders than bacterial isolates.

Keywords : Azo dye, Sanganer, congo red, methyl orange, methyl red, biodegradation.