Journal of Pure and Applied MicrobiologyVol. 7 No. 1

Prolonging Living Tissue and Organ in Dry State through Anhydrobiosis

Wenxin Geng1, Wei Yang1, Rong Zhang2, Moqing Wang3, Wei Liu1, Huicong Du1, Yangyang Wang1 and Fulin Chen1*

1Provincial Key Laboratory of Biotechnology, Institute of Life Science, Northwest University, Xi?an 710069, China. 2Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi?an 710032, P.R.China. 3Department of Anatomy and Cell Biology, Faculty of Biomedical, Biological Science, Mcgill university, 845 Rue Sherbrooke, Montreal, Canada H3A2T5.

Received on 10 January 2013 and accepted on 24 February 2013

 

ABSTRACT

To survive in desiccation, a phenomenon known as ?anhydrobiosis? is widely used by some simple organisms, such as water bear and larvae of sleeping chironomid. From previous experience, we know that the ability to survive in anhydrobiosis or in dry state is correlated with the accumulation of trehalose. Recently, several new reports have demonstrated that endogenous and exogenous trehalose has also been used to increase desiccation tolerance of mammalian cells. Herein, we postulate that trehalose can be injected into donated tissue and organ in the dry state as a method for long-term storage and transportation of liveng tissue and organ. Trehalose can be introduced into cells of tissue and organ through the trehalose-containing perfusion medium. These trehalose-loaded tissue and organ can be dried and stored at room temperature under vacuum.

Keywords : Trehalose, Drying preservation, Anhydrobiosis.