Journal of Pure and Applied MicrobiologyVol. 7 No. 1

Isolation and Genera Distribution of Hydrogen-oxidizing Bacteria in Loess Plateau Hippophae rhamnoides Rhizosphere

Lin Wang1, Bo Fu1, 2, Xin Guo1, Guilin Cao1 and Weiwei Wang1*

1 Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi?an, China. 2 Enzyme Engineering Institute, Shaanxi Academy of Sciences, Xi?an, China.

Received on 20 January 2013 and accepted on 02 March 2013

 

ABSTRACT

Rhizosphere of Hippophae rhamnoides was designated as research material. 39 bacterial strains were isolated by mineral salt agar (MSA) medium with a continuous H2 supplying gas-cycling incubating system. Hydrogenase-positive strains were screened by both 2,3,5-triphenyltetrazolium chloride (TTC) and H2 oxidizing capability tests. 6 strains with positive TTC result and strong H2 oxidizing capability were determined to be hydrogen-oxidizing bacteria. The 6 strains were classified into genera of Bacillus sp, Aeromonas sp, Pseudomonas sp and Micrococcus sp by their cultural, morphology, physiological and biochemical characteristics. 16S rDNA sequence of strain FS2 has a homology of 99% with Bacillus sp and they are in the same branch of phylogenetic tree, indicating strain FS2 belongs to Bacillus sp.

Keywords : Hippophae rhamnoides, Symbiotic nitrogen-fixing, Hydrogen-oxidizing bacteria.