Journal of Pure and Applied MicrobiologyVol. 7 No. 1

Evaluation of Soil Amendment with Three Plants on Fusarium spp. Isolated from Carnation Plant

Abeer R.M. Abd El-Aziz1*, Mona M.M. Ragab2, S. El-Morsy3, Monira R. Al-Othman1, Mohamed A. Mahmoud1,3 and Saleh A. Al-Sohaibani1

1Botany and Microbiology Department, College of Science, King Saud University, Riyadh 1145, Kingdom of Saudi Arabia. 2Faculty of Agricultural, Plant Pathology Department, Cairo University, Giza, Egypt. 3Plant Pathology Research Institute, ARC, Giza, Egypt.

Received on 12 September 2012 and accepted on 20 October 2012



Soil amendment for controlling three Fusarium species, Fusarium oxysporum (Sehlect. Emend Snyd. & Hans), F. solani (Mart.) (Apple and Wr. Emend. Snyd. & Hans) and F. moniliforme (Sheldon) were isolated from carnation. Cuttings were planted in soil artificially infested with the pathogenic fungi tested and amended singly with powder dry material of Eucalyptus, Leek or Thyme to study their effect on % infection and some growth parameters in soil infested with the pathogenic tested fungi of fusarium spp. under greenhouse conditions. The possible biochemical changes (phenolic compounds, oxidative enzymes and chlorophyll and carotene contents) associated soil amendment with three tested dry plants for controlling the disease in the present study was investigated. Herb of thyme was amended with soil proved to be the most effective one in the prevention of infection in case of infested with any three tested fungi while leek the least one. Also, fungicide (Topsin M-70) was tested and other commercial bioproduct Plant-Guard (original component Trichoderma harzianum). Mixing infested soil with tested powder materials lead to increase levels of phenolics compounds, oxidative enzymes and chlorophyll and carotenoides contents compared with those obtained from non treated cuttings in infested soil.

Keywords : Soil amendment, fusarium, Carnation, Biochemical studies.