Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Antifungal Activity of the Native Essential oil of Thymus vulgaris on Candida albicans, Aspergillus niger and Aspergillus flavus from Iran

N. Rahimifard, Sh. Shoeibi et al.

¹Food and Drug Laboratory Research center (FDLRC), Tehran, Iran. ²Food and Drug Control Laboratories (FDCLs), Ministry of Health (MOH), Tehran, Iran. ³Pharmacy faculty, Tehran University of Medical Sciences (TUMS), Tehran, Iran. 4Immunology Department, Pasteur Institute, Tehran, Iran. 5Microbiology Department, Pharmaceutical sciences branch, Islamic Azad University, Tehran, Iran.

Received on 11 April 2008 and accepted on 08 August 2008

 

ABSTRACT

The composition of the native essential oil of Thymus vulgaris and its antifungal activity on Candida albicans, Aspergillus niger and Aspergillus flavus fungal strains were studied in Iran. Essential oil from the aerial parts of the plant was obtained by hydrodistillation and analysed by GC and GC-MS. The oil showed high contents of carvacrol and thymol. The MIC was used to evaluate the antifungal activity against Candida albicans ATCC 10231, Aspergillus niger ATCC 9642 and Aspergillus flavus ATCC 9643. Antifungal activity was evaluated for the essential oil and simultaneously for Amphotricin B. Results showed that Thymus vulgaris essential oil exhibited a significant activity against fungi, and its MIC on Candida albicans, Aspergillus niger and Aspergillus flavus were respectively 0.30, 0.15 and 0.25 µg ml-1 (ppm). The present study indicates that Thymus vulgaris essential oil has considerable antifungal activity, deserving further investigation for clinical applications.

Keywords : Thymus vulgaris, MIC, Antifungal activity.