Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Extraction, Purification and Characterization of Polyphenol Oxidase from Tobacco Grown in Northern Iran

Reyhaneh Sariri¹*, Zahra Mozafarzadeh¹ and Vahab Jafarian²

¹Department of Biology, Faculty of Science, Gilan University, Rasht, Iran. ²Department of Biology, Islamic Azad University, Zanjan Branch, Zanjan, Iran.

Received on 10 June 2008 and accepted on 25 August 2008



Polyphenol oxidase plays a key role in plant defense systems. Screening of tobacco (Nicotiana tobacum) leaves grown in Northern Iran demonstrated that there was a polyphenol oxidase with some similarities with polyphenol oxidases from other sources. Using the fresh leaves of the plant as a source, the polyphenol oxidase content was isolated, purified and characterized. The specific activity of polyphenol oxidase was increased at various stages of purification process. Its optimum pH and temperature were similar to those reported for polyphenol oxidase from citrus grown in other parts of the world.

Keywords : Polyphenol oxidase, Nicotiana tobacum, tobacco, enzyme extraction, purification.