Journal of Pure and Applied MicrobiologyVol. 7 No. 1

Analysis of the Genetic Diversity and Relationship within Hypsizygus marmoreus Based on Sequence - Related Amplification Polymorphism

Chengshu Qiu, Wenjuan Yan, Bin Song, Peng Li, Wangqiu Deng and Taihui Li

1School of Bioscience & Bioengineering, South China University of Technology, Guangzhou - 510 006, China, 2Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology (Ministry?Guangdong Province Jointly Breeding Base) South China, Guangdong Institute of Microbiology, Guangzhou - 510 070, China.

Received on 24 September 2012 and accepted on 08 November 2012

 

ABSTRACT

Germplasm resources of Hypsizygus marmoreus were collected from different parts of China. Sequence-related amplified polymorphism (SRAP) markers were employed to evaluate the genetic diversity and relationship among the 32 collected strains of H. marmoreus. Fifty-five SRAP primer combinations were used and 533 polymorphic bands were observed. Phylogenetic analysis by Unweighted Pair Group Method with Arithmetic Mean based on similarity matrices indicated three clusters, namely, A, B, and C. The Jaccard?s similarity coefficients of genetic similarity among all accessions ranged from 0.40 to 0.73, and averaged 0.50 among these strains collected in China, revealing a medium level of genetic diversity. The results demonstrated that SRAP can be used to evaluate the genetic diversity of H. marmoreus in China and to distinguish the different storage and cultivar strains. These applications are helpful for the effective evaluation, management, utilization, and conservation of germplasm resources.

Keywords : Germplasm source, evaluate, Unweighted Pair Group Method with Arithmetic Mean (UPGMA), China.