Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Characterization and Identification of Thermophilic Lipase Producing Bacteria from Thermogenic Compost

Fida Madayanti, Baiq Vera El Viera, Made Puspasari Widhiastuty and Akhmaloka*

Biochemistry Research Division, Faculty of Mathematic and Natural Sciences, Institute Teknologi Bandung, Jln Ganesha 10, Bandung. Indonesia.

Received on 24 June 2008 and accepted on 08 September 2008

 

ABSTRACT

Thermophilic lipase producing microorganisms were isolated from Pasir Impun thermogenic compost at temperature between 50-70o C. From 40 isolates tested, 10 isolates showed lipolytic activity on agar plate media containing olive oil and rhodamine B. The isolates showed lipolytic activity in the range of 15 315 U/L on p-nitrophenyl palmitate (pNPP) as substrate. Two isolates, namely PI6A and PI13C, were further characterized and showed maximum specific activity at a mid exponential of growth phase. Identification of the isolate was performed through ribotyping method. Chromosomal DNA from these isolates was extracted and the 16S rRNA gene fragments were amplified, resulting fragment length at around 1.5 kb on agarose gel. Further analysis of the 16S rRNA gene sequences using homological and phylogentetical methods showed that the PI6A and PI13C were close to Bacillus licheniformic and Bacillus subtilis respectively.

Keywords : Lipase, thermophiles, 16S rRNA, compost, Pasir Impun.