Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Ver-1 Gene Sequencing and Homology of Two Egyptian Aspergillus Isolates

Mohamed Mokhtar Mohamadein1*, Rasha Mohamed Farrag2 and Eman Ahmed Helmy3

1-3Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt. 1Faculty of Applied Medical Science, King Salman Ben AbdelAziz University, Al-Kharj, KSA.

Received on 18 September 2012 and accepted on 23 November 2012

 

ABSTRACT

Sequencing of the conserved region of ver-1 gene in each of two Egyptian Aspergillus sp., A. nidulans and A. parasiticus isolates, was conducted. Variation in DNA size (515 bp for A. nidulans and 495 bp for A. parasiticus) was observed with a high homology degree of 97%, however with no difference in the translated amino acid sequence. The obtained ver-1 gene sequence of both isolates was matched, using genebank database, with that of each of a no. of strains belonging to Aspergillus species, protein homology with the most similar strain was then conducted. No difference in the translated amino acids was observed for ver-1 gene of the A. nidulans isolate while two amino acid variations were observed in case of A. parasiticus isolate. The obtained sequences of the two investigated local isolates revealed no matching results with any of the A. nidulans strains on the gene bank database reflecting the uniqueness of the A. nidulans Egyptian isolate.

Keywords : Aspergillus sp.; Conserved region; Ver-1 gene; Homology.