Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Extracellular Keratinolytic Proteases from An Alkalophilic Streptomyces albidoflavus TBG-S13A5: Enhanced Production and Characterization

S. Indhuja1, S. Shiburaj1, N.S. Pradeep1, V. Thankamani2 and T.K. Abraham3

1Molecular Taxonomy Laboratory, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala - 695 562, India. 2Department of Biotechnology, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala - 695 581, India. 3Department of Biotechnology and Biochemical Engineering, Mohandas College of Engineering and Technology, Anad, Nedumangad, Thiruvananthapuram, Kerala - 695 544, India.

Received on 08 March 2012 and accepted on 14 May 2012

 

ABSTRACT

An alkalophilic strain of Streptomyces albidoflavus TBG-S13A5 secreted keratin hydrolysing proteases in submerged batch cultures in basal broth of pH 10.5 at 30±20C with white chicken feathers as inducer substrate. Enhanced protease production of around six fold was achieved with modified nutritional parameters viz., sodium nitrate (0.24%), magnesium sulphate (0.03%) and feather (0.8%). Under solid state fermentation maximum protease production was on 7thday (~52 U/gds). The protease activity of the crude enzyme was studied in relation to various factors. The crude enzyme was active and stable in neutral and alkaline conditions. Maximal protease activity was at pH 9.0 and in the temperature range of 60-700C. Protease activity was partially inhibited individually by the protease inhibitors PMSF (Phenylmethylsulfonyl fluoride) and EDTA (Ethylene diamine tetra aceticacid). The crude enzyme was generally tolerant to the detergents and solvents tested. Protease activity was partially inhibited by the reducing agent, b-Mercaptoethanol(b-ME). Metal ions like BaCl2, MgCl2 and MgSO4 increased protease activity whereas it was strongly inhibited by HgCl2, ZnSO4 and CuSO4.

Keywords : Streptomyces albidoflavus, Keratin hydrolysing proteases, Solid state fermentation.