Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Screening of Chitinase Producing Strain and Effect of its Metabolite on Magnesium Ammonium Phosphate Crystals Fractal

Guangjun Nie*, Wenjin Yue, Hongtao Fang, Yan Jiang and Yongliang Shen

Department of Biochemical Engineering, Anhui University of Technology and Science Wuhu - 241 000, China

Received on 06 April 2008 and accepted on 15 August 2008

 

ABSTRACT

Chitinase producing microorganism was obtained from soil rich in chitin, and its metabolite was separated and purified by the method of salting out and chromatography respectively. Capillary electrophoresis was used to determine the homogenization of chitinase. The result showed that chitinase is homogenous to a certain extent, and its corresponding concentration is about 1.7145mg/ml. The method of double diffusion was employed to study the fractal of magnesium ammonium phosphate crystals (MAPs), and different concentration of chitinase solutions were added into reactive system of MAPs formation in order to investigate effect of chitinase on MAPs fractal. The results indicated that with the increasing of chitinase concentration, initial time of MAPs formation becomes longer and longer, crystal shape smaller and smaller, crystal mass less and less. It is suggested that chitinase could inhibit MAPs formation. MAP was characterized by XRD. The crystal is mainly magnesium ammonium phosphate hexahydrate (MgNH4PO4.6H2O) and a little magnesium ammonium phosphate hydrate (MgNH4PO4. H2O).

Keywords : Chitinase, MAPs, Fractal, Screening, XRD.