Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Evaluation of Physical Parameters Involved in Mycoremediation of Benzo[a]Pyrene by Pleurotus ostreatus

Sourav Bhattacharya1*, J. Angayarkanni2, Arijit Das3 and M. Palaniswamy1

1Department of Microbiology, Karpagam University, Coimbatore - 641 021, India. 2Department of Microbial Biotechnology, Bharathiar University, Coimbatore - 641 046, India. 3Department of Microbiology, Genohelix Biolabs, A Division of Centre for Advanced Studies in Biosciences, Jain University, 127/2, Bull Temple Road, Chamarajpet, Bangalore - 560 019, India.

Received on 04 May 2012 and accepted on 08 June 2012

 

ABSTRACT

Benzo[a]pyrene (BaP) is a highly toxic organic pollutant widely distributed in terrestrial and aquatic environments and is often referred as a xenobiotic compound. The present investigation was carried out to determine the physical factors affecting the biodegradation of BaP by the PO-3 isolate of Pleurotus ostreatus. When tested for the effect of media pH on the extent of BaP degradation, pH 6.0 facilitated both highest degradation (63.1%) and fungal biomass accumulation. Mesophilic range of temperature in general supported good fungal growth and subsequent degradation of the toxin. However, the highest degradation (64.2%) was observed when the inoculated media was incubated at 30°C. Subjecting the mineral salt broth to different agitation speeds showed different levels of BaP removal, wherein 180 rpm resulted in the highest level of BaP degradation (66.3%). Eventually, increasing the agitation speed beyond this decreased both the biomass and the extent of BaP degradation. Thus, the biodegradative potential of Pleurotus ostreatus PO-3 is strongly affected by these physical factors. A better understanding of the other parameters may result in effective decontamination of the polluted sites using Pleurotus ostreatus PO-3 isolate.

Keywords : Benzo[a]pyrene, Pleurotus ostreatus, Degradation, pH, Temperature, Agitation.