Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Strain Improvement of Fresh Water Actinomycetes for Increased Antibacterial Metabolite Production

Madan Mohan Gunda1* and M.A. Singarachraya2

1Department of Industrial Microbiology, S.R.R. Govt. Degree & P.G College, Karimnagar, India. 2Department of Microbiology, Kakatiya University, Warangal, India.

Received on 08 April 2012 and accepted on 12 May 2012

 

ABSTRACT

A total of twenty four actinomycetes strains were isolated from three fresh water systems of Karimnagar, Andhra Pradesh and their antibacterial activities were tested against ten test bacteria namely Bacillus subtilis (MTCC 441), Proteus vulgaris (MTCC 426), Staphylococcus aureus (MTCC 96), Pseudomonas aeruginosa (MTCC 424), Enterobacter aerogenes (MTCC 111), Salmonella typhi (MTCC 733), Escherichia coli (MTCC 40), Sarcina lutea (MTCC 1541), Shigella flexneri (MTCC 1457) and Klebsiella pneumonia (MTCC 7162). Among, eight strains showed good antibacterial activity. Two of these eight isolates (LAM1 and LAM2) were found to produce wide spectrum of antibacterial substances which were selected for mutation study using physical (U.V radiation and X-rays) and chemical (EtBr and NTG) mutagens. After the mutations, antibacterial activity of the strains, LAM1, LAM2 (U.V treated), LAM1 (X-ray treated) and LAM1, LAM2 (EtBr and NTG treated) was increased but the LAM2 (X-ray treated) strain showed decreased antibacterial activity.

Keywords : Actinomycetes, Fresh water, Bacteria, Mutation.