Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Protein Enrichment of Wastes from Three Nigerian Yam Varieties using Mono and Co-cultures of Amylolytic Fungi

O. Emmanuel Nwafor

Department of Microbiology, Delta State University, Abraka, Nigeria.

Received on 12 June 2012 and accepted on 30 July 2012

 

ABSTRACT

Protein enrichment of wastes from Dioscorea rotundata, Dioscorea cayenensis and Dioscoreaalata was investigated by solid state fermentation using mono and co-cultures of four amylolytic fungi. The wastes provided the sole carbon source while mineral elements were from (NH4)SO4, urea and KHPO4. Fermentation was allowed to progress for 5days. pH, moisture and protein contents were assessed on the first day and on the 5th day for the various yam varieties. Protein contents increased from the value of 3.820.29% (w/w) to values that ranged from 8.460.30% (w/w) from S. cerevisiae on D.rotundata to 10.100.50% (w/w) from Mucor sp on D.alata. Addition of booster doses of nitrogenous supplements resulted in further increases in protein yield. The use of co-cultures of the fungi showed a further increase in protein although not up to double for the values obtained from those where mono cultures were inoculated. There were decreases in pH values with increasing moisture contents. Results indicate the possibility of the use of the bioconverted wastes as feeds for poultry and other animals after appropriate toxicological studies.

Keywords : Protein, Enrichment, Mono, Co-cultures, Amylolytic fungi.