Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Adhesion of Escherichia coli to Glass Under Different pH

Fatima Hamadi, Hassan Latrache et al.,

¹Equipe de recherche: Microbiologie et biochimie appliquee a l’agroalimentaire, l’environnement et la sante. Facultedes Sciences et Techniques, B.P 523, Beni Mellal, MAROC ²Departement Securite Alimentaire & Environnementale. Institut Pasteur. Maroc ³Laboratoire de T?l?d?tection et des Systemes d’Information G?ographique Appliques aux  Geosciences et ? lEnvironnement. Faculte de Sciences et Techniques, B.P 523, Beni Mellal, MAROC. 4Laboratoire de physique des materiaux. Facult? de Sciences et Techniques, B.P 523, Beni Mellal, MAROC.

Received on 20 April 2008 and accepted on 24 May 2008

 

ABSTRACT

The hydrophobic/hydrophilic and electron donor/electron acceptor characters for Escherichia coli AL52 were determined using microbial adhesion to solvents method (MATS). The glass with adhered cells was observed using scanning electron microscope (SEM) and the SEM images were processed with Mathlab? program to quantify the adhesion to glass. Strong adhesion was found at pH 2. For pH 3 the adhesion was relatively high but for other pH it was low or null. The results also show that the electron donor character plays a crucial role in microbial adhesion of Escherichia coli to glass. Moreover the role of hydrophilic-hydrophilic interactions is not always sufficient to explain adhesion between two hydrophilic surfaces. Also our finding shows that the types of interactions governing microbial adhesion to glass depend on pH of suspending medium.

Keywords : Escherichia coli, Adhesion, pH, electron donor character, hydrophobicity.