ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Hawraa F. Wali , Yasir Adil Jabbar Alabdali and Mohammed Qasim Waheeb
Department of Biology, College of Science, Al Muthanna University, AL-Muthanna, Iraq.
J Pure Appl Microbiol. 2020;14(2):1519-1525 | Article Number: 6095
https://doi.org/10.22207/JPAM.14.2.50 | © The Author(s). 2020
Received: 18/02/2020 | Accepted: 27/04/2020 | Published: 10/06/2020
Abstract

Silver nanoparticles (AgNPs), have been widely used as antibacterial therapy for any microorganisms that are multidrug resistance to antibiotics. Pseudomonas aeruginosa is the most common respiratory pathogen in patients with cystic fibrosis (CF), was collected from Al-Muthanna hospitals. These isolates were drug-resistant against tetracycline, chloramphenicol, erythromycin, streptomycin, azithromycin and trimethoprim, while they were sensitive to imipenem. The conventional PCR was used to screen for many different virulent genes and eventually, the only algD and plcH were detected among 11 Pseudomonas aeruginosa strains over of twenty isolates of P. aeruginosa isolated from patients with cystic fibrosis (CF) disease. The Ag/F Tio2 NPs was used as antibacterial to test the AgNPs activity against the expression of algD and plcH genes that are screened as a common complication of gene virulence in the cystic fibrosis (CF) disease. The results showed a significant effect on algD and plcH genes expression of P. aeruginosa.

Keywords

Pseudomonas aeruginosa, Ag/F Tio2, Cystic Fibrosis, Nanoparticles

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