ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Muhammad Rizwan1, Said Amin2, Bates Kudaibergenova Malikovna3, Abdur Rauf4, Muhammad Siddique5, Kamran Ullah6, Saud Bawazeer7, Umar Farooq8, Yahia Naseer Mabkhot9,10 and Mohamed Fawzy Ramadan11,12
1Centre for Biotechnology and Microbiology, University of Swat, KPK, Pakistan.
2Department of Microbiology and Biotechnology, Abasyn University Peshawar, Pakistan.
3Department of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
4Department of Chemistry, University of Swabi, KPK, Pakistan.
5PCSIR Complex Peshawar, KPK, Pakistan.
6Department of Zoology, University of Swabi, KPK, Pakistan.
7Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, Makkah, P.O. Box 42, Saudi Arabia.
8Department of Chemistry, University of COMSATS, Abbottabad, Pakistan.
9Department of Pharmaceutical Chemistry, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
10Research Center for Advanced Materials Sciences (RCAMS), King Khalid University, Abha-6113, Saudi Arabia.
11Deanship of Scientific Research, Umm Al-Qura University, Makkah, P.O. Box 715, Saudi Arabia.
12Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig 44519, Egypt.
J Pure Appl Microbiol. 2020;14(2):1437-1451 | Article Number: 6418
https://doi.org/10.22207/JPAM.14.2.42 | © The Author(s). 2020
Received: 02/04/2020 | Accepted: 05/06/2020 | Published: 20/06/2020
Abstract

The goals of the present study were to use silver nitrate (AgNO3) solution to synthesize plant-mediated silver nanoparticles (AgNPs) using Boerhavia procumbens extract, to evaluate the antimicrobial potential of crude B. procumbens extracts as well as the antimicrobial potential of synthesized AgNPs. The antimicrobial activity was tested against ten pathogenic bacterial strains including Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Citrobacter braakii, Providentia spp., Salmonella typhi, Salmonella para typhi, Vibrio cholera, and Proteus vulgaris and seven fungal species; Rhizopus stolonifer, Candida albican, Alternaria alternata, Aspergillus flavus, Verticillium chlamydosporium, Penicillium chrysogenum, and Aspergillus oryzae. The methanol extract was fractionated using several solvents and subjected to phytochemical analysis along with FTIR. Phytochemical analyses revealed flavonoids, tannins, saponins, steroids, quinones, and phenols in the crude plant extract. AgNPs were synthesized using B. percumbens extract and characterized by UV-Vis, Fourier-transform infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). Synthesized AgNPs were spherical, with 20-80 nm diameter. The absorption peak of synthesized AgNPs was observed at 392 nm. AgNPs have significant antimicrobial potential against selected pathogenic bacterial and fungal species as compared to different fractions of crude B. procumbens extract. The current study suggests that green synthesis is a useful technique and can be used as an alternative to antimicrobial agents against pathogenic organisms.

Keywords

Boerhavia procumbens, Nyctaginaceae, scanning electron microscopy, FTIR, silver nanoparticles, Quinones

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