ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
P. Arun Kumar, Roma A. Chougale and Indireddy Sinduri
Department of Microbiology, D.Y. Patil Medical College, Kolhapur – 416 005, Maharashtra, India.
J Pure Appl Microbiol. 2020;14(2):1405-1411 | Article Number: 6180 | © The Author(s). 2020
Received: 22/03/2020 | Accepted: 24/05/2020 | Published: 25/06/2020

To minimize exposure to hazardous biological materials, all doctors, nurses, technicians and in general all the healthcare personnel as well as researchers wear a knee-length, long-sleeved, elastic-cuffed laboratory coat while working with hazardous materials. A white coat provides a protective layer and can be easily removed if contaminated. White coats act as mechanical vectors in transmission of pathogenic and non-pathogenic bacteria to the people who are associated with health care workers. A total of 120 specimens were collected by using sterile cotton swabs. Of these, 88 (73%) white coats were contaminated. At the same time, a pre-validated and semi-structured questionnaire (containing questions regarding usage of white coat, purpose, hygiene, and perception about contamination of white coats, etc) was distributed. Gram positive cocci 64 (72.72%) were isolated more than gram negative bacilli 24 (27.28%). Most of the tested antibiotics showed resistance to isolated gram positive and gram negative bacteria. 10 (41.66%) isolates of gram negative bacilli were resistant to extended spectrum beta-lactamases (ESBL) and 10 (6.4%) of staphylococcal isolates showed Methicillin resistance. Therefore, a much more attention to wear a clean white coat should be required.


White coat, ESBL, MRSA, Gram negative bacilli, Gram Positive cocci

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.