ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sumardi , Salman Farisi, Christina Nugroho Ekowati, Achmad Arifiyanto and Dwi Eka Rahmawati
Department of Biology, Faculty of Mathematics and Natural Sciences University of Lampung, Jl. Sumantri Brojonegoro No. 1. Bandar Lampung, Lampung, 35145, Indonesia.
J Pure Appl Microbiol. 2020; 14(2):1237-1244 | Article Number: 6179 | © The Author(s). 2020
Received: 21/03/2020 | Accepted: 12/06/2020 | Published: 20/06/2020

Mannose and mannooligosaccharide acted as prebiotic that consumed by probiotic bacteria. Mannanase were the second most important enzymes for the hydrolysis of hemicelluloses, beside xylanase. The objective research was to obtain eminent strains of Bacillus sp. in mannan degradation which can potentially be a probiotic candidate. The study employed a completely randomized design using four concentration levels repeated six times. Halotolerant Bacteria were isolated from mangrove ecosystem at Hanura beach, Teluk Pandan, Pesawaran District in Lampung Province. They are grown on the sea water complete agar media. Mannan degradation isolates were then characterized to determine their character with a variety of tests, including resistance to pH, salt, and metal ions, pathogenicity, and determination of mannanase production duration. Thirty strains are found to grow at 3-6% salt content, and 9 of them have mannanolytic activity. They grow optimally at pH 7-10. Seven isolates were proven to be positively hydrolyzed blood agar in the pathogenicity test. The addition of Iron (III) Chloride increased the enzyme activity by 11.12% in IBK3 isolates at 96 hours of cultivation period which was 0.05 UmL-1. It acted as cofactors of enzymatic reactions. Strains Bacillus sp., were able to degrade mannan substrate. It quantified using Index of mannanolytic. Strain IBK3 has the highest index of mannanolytic activity as much as 10.74. Their ability to grow in salt media indicated that they were halotolerant. They were more likely to live at base rather than acid habitat. Only IBK3 and ID2K1 showed non-pathogenic isolates. Only FeCl3 addition has proven to rise up enzymatic activity.


Characterization, Bacillus sp., Mannanase, Probiotic

Article Metrics

Article View: 253
PDF Download: 47

Share This Article

© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.