ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Pooja Sharma1, Sonam Tripathi1, Preeti Chaturvedi2 and Ram Chandra1
1Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar Central University, Vidya Vihar, Rae Bareli Road, Lucknow – 226 025, India.
2Aquatic Toxicology Laboratory, Environmental Toxicology Group, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhavan, 31, M.G. Marg, Lucknow – 226 001, India.
J Pure Appl Microbiol. 2020;14(2):1181-1194 | Article Number: 6083
https://doi.org/10.22207/JPAM.14.2.13 | © The Author(s). 2020
Received: 11/02/2020 | Accepted: 05/05/2020 | Published: 10/06/2020
Abstract

The purpose of this study is remediation of residual organic pollutants from effluent by autochthonous bacterial community in biostimulation process. Discharged effluent showed high TDS (549 mg L-1), TSS (59 mg L-1), COD (20349 mg L-1) and BOD (25946 mg L-1), value. The level of total phenol (421 mg L-1), nitrogen (156 mg L-1), sulphate (1854 mg L-1), phosphorus (176 mg L-1) chlorine (2.01 mg L-1), sodium (75 mg L-1) and potassium (8.4 mg L-1) along with various heavy metals (Fe, 75.23; Zn, 15.60; Cu, 4.1; Cr, 3.12; Cd, 0.324; Mn, 13.24; and Ni, 4.01 mg L-1) were noted above the permissible limit of Environmental Protection Act. The result revealed that the reduction of the physico-chemical parameter of pollutants were above 50% after biostimulation process, this confirmed the potentiality of growing autochthonous bacterial community responsible for bioremediation. The comparative UV-Vis spectroscopy showed reduction in the absorption spectra of degraded sample. Further, GC-MS analysis showed major organic pollutants i.e. Octadecanoic acid, Hexadecanoic acid, citral, benzoic acid, and 2, 6’-Di-hydroxy acetophenone, bis (trimethylsilyl) ether, were detected in control few compounds were degraded while there was formation of some new metabolic products also. Few pollutants persisted in the degraded sample as recalcitrant toxicant and causes environmental toxicity and hormonal imbalance as endocrine-disrupting chemicals (EDCs). But the detailed knowledge and characterization of organic pollutants are not available yet regarding their properties. The SEM image showed the diversity of bacterial community in biostimulation responsible for utilisation of various detected compounds. The growing bacterial communities were identified as potential bacterial strains as Aeromonas salmonicida, BBAUPS-1 (MN294457.1) and Bacillus sp. BBAUPS-2 (MN238724.1) responsible for the remediation of residual organic pollutants. Further, Evaluation of toxicity parameter of effluent by seed germination test of Triticum aestivum and Cicer arietinum inhibited the seed germination upto 80%. Hence, this study revealed that the biostimulation process is a good technique for detoxification and degradation effluent.

Keywords

Environmental toxicity, endocrine-disrupting chemicals, organic pollutants, phytotoxicity, effluent detoxification

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