ISSN: 0973-7510

E-ISSN: 2581-690X

Smriti Shrivastava1, Vishal Kumar2, Mehak Baweja2 and Pratyoosh Shukla2
1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector 125,
NOIDA, Uttar Pradesh, 201303, India.
2Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak-124001, Haryana, India.
J. Pure Appl. Microbiol., 2016, 10 (3): 2225-2230
© The Author(s). 2016
Received: 13/01/2016 | Accepted: 19/04/2016 | Published: 30/09/2016
Abstract

Extracellular xylanase production by Thermomyces lanuginosus (NCIM-1374/ DSM 28966) could be enhanced using statistical tools of Response surface methodology. Optimized process parameters (pH: 6.3; temperature: 52°C; fermentation time: 108 h; substrate concentration: 1.8% wheat bran) were obtained using ANNOVA. Saccharification process under optimized condition enhanced extracellular xylanase production from 10012 IU/L/min to 12060 IU/L/min. Experimental design showed high correlation between predicted and experiment R-squared values, and analysis of variance had computed F-value of 16.57 with a very low P-value indicating the statistical significance of the quadratic model that can be used to navigate the design space with an adequate precision measure and could optimize the process for high level xylanase production. Thermomyces lanuginosus NCIM1374/ DSM 28966 is an indigenous strain isolated in our laboratory and its xylanase has unique capacity of hydrolyzing xylan to xylose. In the present study we were able to enhance extracellular production of this swift and differently acting enzyme.

Keywords

Thermomyces lanuginosus, Xylanase, Response surface methodology, Quadratic model, Box- Behnken Design.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.