ISSN: 0973-7510

E-ISSN: 2581-690X

Sargol Aminnezhad1, Rouha Kasra Kermanshahi1 and Reza Ranjbar2
1Department of Biology, School of Science, Alzahra University, Tehran, Iran.
2Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
J. Pure Appl. Microbiol., 2016, 10 (3): 1857-1863
© The Author(s). 2016
Received: 13/01/2016 | Accepted: 19/04/2016 | Published: 30/09/2016
Abstract

Pseudomonas aeruginosa is an important opportunistic pathogen that typically produces the biofilm. Biofilm- producing bacteria are inherently resistant to chemical treatment. This study examines the effect of Althaea officinalis extract on the biofilm formation by Pseudomonas aeruginosa. Antibiotic susceptibility test of Pseudomonas aeruginosa was performed by disk diffusion method and the effect of alcoholic extract of herb marshmallow (Althaea officinalis) was studied on bacteria. Hock extract collected from Northwest of Iran (Kurdistan) was prepared using soxhlet extractor. Antimicrobial properties of the extract were evaluated using well plates. Minimum inhibitory concentration (MIC) and  minimal lethal concentration (MBC) of plant extracts and antibiotics were determined using CLSI standard plate.   Biofilm formation was performed using a modified micro-titer plates. The MIC and MBC (62.5 mg D  ml) showed significant effects of studied plants on the bacteria. Different concentrations of Althaea officinalis extract significantly reduced the biofilm produced  by  Pseudomonas  aeruginosa  in  comparison  with  the  positive  control  (P  value  = 0.0124). According to our findings, significant effect of this natural substance can be a promising agent in the treatment of Pseudomonas aeruginosa infection; and also an inhibitor of biofilm formation.

Keywords

Althaea officinalis extract, Biofilm formation, Pseudomonas aeruginosa.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.