Journal of Pure and Applied MicrobiologyVol. 11 No. 4

Study of Erythromycin Resistance genes in Staphylococcus aureus Isolated from Throat Swab Culture

Halemah Mohamed Abulkasim*, G.S. Shukla, H.K. Bajaj and Harrison Masih

Department of Clinical laboratory Sciences-Faculty of Health Science, SHUATS, Allahabad-211007. U.P., India.

Received on 01 October 2017 and accepted on 30 November 2017

 

ABSTRACT

Throat swabs were collected to isolate S. aureus by using blood agar media. Biochemical tests were performed. Genomic DNA was isolated and PCR was done for erm A, ermB and erm C genes followed by bioinformatics analysis. Isolates were screened on erythromycin disc (erythromycin range 1- 125mg/l). All 47 S. aureus isolates found to be resistance to erythromycin by phenotypic methods contained at least 1 were erythromycin resistance gene. Thatís mean all S. aureus isolates which were resistance to erythromycin by disc diffusion method were found as positive by PCR for erm A, B, and C genes.

Keywords : Throat swabs, bacteria,ermA, ermB, ermC.