Journal of Pure and Applied MicrobiologyVol. 11 No. 4

Gold Nanoparticles and Laser as Antimicrobial Agents against Some Types of Bacteria

Maysa S.M. Al-Shukri

Department of Microbiology, College of Medicine, Babylon University, Hilla, Iraq .

Received on 10 September 2017 and accepted on 05 November 2017

 

ABSTRACT

The goal of this study was to assess the efficacy of gold nanoparticles (AuNPs) and AuNPs– Low level laser combined therapy as antibacterial agents against different types of bacterial isolates in vitro. Five concentrations of AuNPs (25,50,75, 100 and 200 ìg/mL) were employed for assessing bacterial growth rate and the bacterial MIC. It was found that there was slight reduce in bacterial growth after treatment with gold nanopracticle. The influence of gold nanoparticles on Staphylococcus aureus biofilms was also studied. It was found that biofilm growth was decreased at different concentrations of AuNPs compared to absence of nanoparticles. Results of the cytotoxic act of AuNPs and low level laser effect in combination against Escherichia coli and S. aureus referred that the low level laser light improved the antibacterial action of AuNPs when it used in combination with low level laser.

Keywords : Gold nanoparticles, Laser, Antibacterial agent, Cytotoxicity, MIC.