Journal of Pure and Applied MicrobiologyVol. 11 No. 4

Phenotypic and Genotypic Identification of Bacteria from Women Breast-Milk and the Feces of their Childs in the Western Region of Algeria

Malika Talhi-Mekhici1*, Bertrand Cornu2, Rahma Talhi- Mehaya1, Djemaia Sahraoui1,Wafaa Dib3, Leila Amel Yazi4, Assia Zemmour1, Saidi-Ouahrani Nadjia6, Mourad Kacem5 and Corinne Vander Wauven2

1Laboratoire de Génétique moléculaire, Département de Génétique Moléculaire Appliquée. Université des Sciences et de la Technologie d’Oran Mohamed Boudiaf (USTO-MB), Algérie. 2Institut de Recherches Microbiologiques JM Wiame, Av. E. Gryson 1, B-1070 Bruxelles, Belgique. 3Laboratoire de Physiologie de la Nutrition et Sécurité Alimentaire, Département de Biologie, Faculté des Sciences, Université d'Oran 1, Ahmed Ben Bella, Algérie. 4Service de Bactériologie, Etablissement Hospitalier Universitaire d’Oran (EHUO), Algérie. 5Département de Biotechnologie, Faculté des Sciences, Université d’Oran 2, Mohamed Ben Ahmed, Algérie. 6Laboratoire de biologie du développement et de la différenciation, Université d’Oran 1, Ahmed Ben Bella, Algérie.

Received on 02 October 2017 and accepted on 07 November 2017

 

ABSTRACT

Breast-milk is an important source of bacteria for the colonization of the infant‘s gut. The aim of our study was to isolate and identify bacteria from samples of breast-milk of 32 women and from fecal samples of their breast-fed infants. Antimicrobial activity of isolates was also performed. A total of 155 isolates were characterized by phenotypic tests and identified by 16S rDNA sequencing analysis. The isolates belonged to 6 different species of acid lactic bacteria (LAB) and Staphylococcus epidermidis. Enterococcus faecium was the most frequently isolated species (40.8%) and faeces (42.5%). According to the mother’s lifestyle, we noticed that the genus Enterococcus was the most frequently isolated from rural mother’s milk as well as urban mother’s milk. Lactobacillus fermentum (P < 0.05) and Staphylococcus epidermidis (P < 0.01) were isolated only from rural mother’s milk. An antimicrobial activity was observed in 30 strains from 148 LAB, the higher level of antagonist was with E.faecium (35 mm). The observed results showed that the isolated strains from rural mother’s milk were different from that urban mother’s milk. Eventual studies can be carried out about lifestyle and nutrition of mothers to explain the effect on the flora found in the milk and feces infants.

Keywords : Human milk, Feces infant, Bacteria, identification, Antimicrobial activity.