Journal of Pure and Applied MicrobiologyVol. 11 No. 3

Optimization of Enzymatic Synthesis of Oleoyl-Diethanolamide in Solvent-Free System

Zuhrina Masyithah1,* and Tjahjono Herawan2

1Department of Chemical Engineering, Faculty of Engineering, Universitas Sumatera Utara. Jalan Almamater, Medan 20155, Indonesia. 2Indonesian Oil Palm Research Institute (IOPRI), Jalan Brigjen Katamso 51, Medan 20158, Indonesia.

Received on 25 June 2017 and accepted on 03 August 2017

 

ABSTRACT

Oleoyl-diethanolamide was synthesized through amidification of diethanolamine (DEA) with oleic acid (OA) using immobile lipase as a biocatalyst. Response Surface Methodology (RSM) based on five-level five variable, Central Composite Rotatable Design (CCRD) was developed to study and to optimize the reaction conditions of oleoyl-diethanolamide synthesis under solvent-free system. The influence of the five main variables namely temperature, reaction time, enzyme amount, substrate molar ratio and step of amine added on amide synthesized was analyzed. Results concluded that quadratic polynomial models is in accordance with the data obtained, with the value of R2 is 0.9846. Although the effect of the gradual addition of the amine is not so significant, but it minimize problems of formation of ion pairs amine/fatty acid highly viscous. Oleic acid converted is 61.35% obtained at the optimum conditions 70 oC, 24 h, enzyme amount 12% (wt/wtOA), three step of amine added and the DEA/OA molar ratio of 7. This is a high ratio of substrate because amine served as a solvent in the reaction. The identity of the oleoyl diethanolamide was confirmed by FTIR. Solvent-free conditions can be used even oleic acid is converted less (61.35%) than synthesis using a solvent (78%).

Keywords : Alkanolamide, Central Composite Rotatable Design, Response Surface Methodology, Solvent-free, Immobile lipase.