Journal of Pure and Applied MicrobiologyVol. 11 No. 3

Impact of Hygiene Intervention Practices on Microbial Load in Raw Milk

Hai Yen Lee1*, Shih Keng Loong1, Jing Jing Khoo1, Fang Shiang Lim1,2, Lay Ching Chai2, Chubashini Suntharalingam4, Jeyabalan Sivalingam5 and Sazaly AbuBakar1,2

1Tropical Infectious Diseases Research and Education Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia. 2Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. 3Institute of Bioscience, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. 4Economics & Social Science Research Centre, Malaysia Agriculture Research and Development Institute (MARDI), 43400 Serdang, Selangor Darul Ehsan. 5MaxVet Enterprise, 19 GF, Jalan BB 1/5, Taman Banting Baru, 42700 Banting, Selangor Darul Ehsan.

Received on 10 June 2017 and accepted on 15 July 2017

 

ABSTRACT

An intervention program was designed to show local dairy farmers on Good Agricultural Practices (GAP) to improve the quality of milk produced by the farms. In this current study, sampling of milk and environmental samples were collected pre- and post-intervention program. Analysis of milk quality was done at field to show the microbiological laboratory procedures to the farmers on determination of milk quality. Findings showed a reduction of up to 40% log reduction in the fresh milk sample. Analysis of the environmental samples were carried out using multiplex MPN-PCR method to quantify and detect up to five species of bacteria in milk. Finding showed the persistence of Salmonella paratyphi contaminating the milking equipment pre- and post-intervention program. The intervention program will be further improved according to data and findings from this study to be implemented at other farms in the future.

Keywords : Milk zoonoses; food safety; hygiene; dairy farms; exposure assessment; Malaysia.