Journal of Pure and Applied MicrobiologyVol. 11 No. 3

Removal of Erionyl blue A-R and Solophenyl Black FR Textile Dyes using Enzymatic Extracts of Laccases of Pleurotus ostreatus and Pleurotus djamor

AIA Alonso-Calderon1, E. Chavez-Bravo2, E. Vidal-Robles3, S. Martinez-Galindo4, J.R. Omaire-Vargas4 and M.A. Marin-Castro5

1Chemical Engineering Faculty, Autonomous University of Puebla, Mexico. University City, Avenue San Claudio and south 18, Colony San Manuel. 2Center for Research in Microbiological Sciences, Institute of Sciences Autonomous University of Puebla, Mexico. 3Chemical Engineering Faculty, Autonomous University of Puebla, Mexico. 4Engineering Faculty, Autonomous University of Puebla, Mexico. 5Agricultural Sciences Research Department of the Sciences-Institute, Autonomous University of Puebla, Mexico.

Received on 30 June 2017 and accepted on 08 August 2017

 

ABSTRACT

In this research, extracellular fungal extracts containing Pleurotus ostreatus and Pleurotus djamor were used for the removal of erionyl blue A-R (anthraquinone) and solophenyl black FR (azo) textile dyes in artificially contaminated water at 200 ppm. For erionyl blue A-R, removals of up to 93.6 and 42.85% were achieved, and for solophenyl black FR of 27 and 31.14%, using the enzymatic extracts of P. ostreatus and P. djamor respectively. Enzymatic activity values of 888.41 IU and 152.22 IU were reached for the laccases obtained from the submerged culture extract of Pleurotus ostreatus and Pleurotus djamor respectively. The extract obtained from P. ostreatus was partially purified using dialysis and anion exchange chromatography (DEAE), by polyacrylamide gel electrophoresis. A molecular mass of 67 kDa was determined.

Keywords : Pleurotus, dye, laccase, enzyme.