Journal of Pure and Applied MicrobiologyVol. 11 No. 2

Variability in Phytophthora drechsleri f. sp. cajani and Effect of Temperature

Chandra Kant Singh1, Ichini Sudhir1, Ramesh Chand1*, Vineeta Singh1 and Mamta Sharma2

1Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India. 2International Crops Research Institute for the Semi-Arid tropics, Patancheru, Hyderabad, 502324, India.

Received on 19 March 2017 and accepted on 01 April 2017

 

ABSTRACT

The present experiment was undertaken to study the cultural, morphological and pathological variation in six isolates of Phytophthora cajani. The isolates were collected from pigeonpea growing areas of eastern Uttar Pradesh. Isolates exhibited considerable variations in cultural characters and growth on potato dextrose agar medium. The universal ITS1 and ITS4 primers showed the difference among P. cajani isolates. There was significance influence of temperature on the growth of isolates, however isolates could not be differentiated based on the temperature. The average growth (1.69 cm2) at 30C temperature was recorded with all the isolates followed by average growth (1.25 cm2) at 35C temperature. The maximum radial growth (3.6 cm2) was observed at 96 h in isolate PDC015-1. The isolate PDC013-1 and PDC014-3 showed highest 47.0% plant mortality on ICP 7119 genotype. Among the isolates PDC014-3 killed 28.6% plants after 4th day of inoculation.

Keywords : Cajanus cajan, Phytophthora Blight, Temperature, Variability.