Journal of Pure and Applied MicrobiologyVol. 11 No. 2

Antibiogram and Serotyping of Vibrio cholerae O1 Isolates from a Tertiary Care Centre in South India

Satish Patil1, Praveen C. Shetty2, Raghavendra D Kulkarni2, Ajantha GS2, Anuradha Kalabhavi2, Deepa Patil2, Manjunath Hosamani2, Pavithra Jain2, A.K. Chakraborti3 and Shubhada C2*

1SS Institute of Medical Sciences and Research Centre, Davangere, Karnataka, India. 2SDM College of Medical Sciences and Hospital, Dharwad, Karnataka, India. 3National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.

Received on 10 February 2017 and accepted on 03 May 2017

 

ABSTRACT

This study was aimed to know the prevalence of biotypes, serotypes and phage types and the antibiotic susceptibility pattern of Vibrio cholerae isolates in our region.1,975 consecutive diarrheal stool samples were processed between 2009 and 2013. Standard microbiological methods and guidelines were followed to isolate, identify Vibrio cholerae and to perform antibiotic susceptibility test. Serotypes, biotypes and phage types of the isolates were determined. 106 Vibrio cholerae O1 strains were isolated from 1,975 stool samples (5.36%). All isolates were El Tor biotype. Predominant serotype was Ogawa (101/106, 95.2%). Phage types T2 (Basu & Mukerjee) and T27 (new scheme) were most common. High level of resistance was seen towards ampicillin and Co-Trimoxazole. Least resistance was towards chloramphenicol. No specific trend was shown against tetracycline. To conclude, V. cholerae O1 El Tor, biotype Ogawa was the most common serotype found in this area. Doxycycline still remains the drug of choice. Antibiotic susceptibility pattern of V. cholerae must be monitored by tertiary care centers.

Keywords : Vibrio cholerae O1, cholera, diarrheal diseases, Serotyping.