Journal of Pure and Applied MicrobiologyVol. 11 No. 2

Isolation and Characterization of Pseudomonas stutzeri as Lead Tolerant Bacteria from Water Bodies of Udaipur, India using 16S rDNA Sequencing Technique

Nadim Chishty1, Garima Verma1 and Chandra Veer2

1Department of Zoology, Government Meera Girls College, Mohan Lal Sukhadia University, Udaipur - 313 001, Rajasthan, India. 2 Department of Dairy & Food Microbiology, College of Dairy & Food Science Technology, MPUAT, Udaipur, Rajasthan, India.

Received on 10 December 2016 and accepted on 10 January 2017

 

ABSTRACT

The present study deals with isolation and characterization of lead resistant bacteria isolated from two water bodies Udaisagar lake and Gadwa pond of Berach river system, Udaipur, Rajasthan, India. Initially, among 13 of the total isolates screened from water samples, 2 isolates were selected for study based on high level of heavy metal resistances. On the basis of morphological, biochemical and molecular characterization using 16S rDNA sequencing the isolates were identified as Pseudomonas stutzeri (KX692284) and Pseudomonas stutzeri (KX692285). The isolates exhibited high resistance to lead (Pb). The Minimum Inhibitory concentration (MIC) of the isolates against lead was determined using agar plate dilution method. Pseudomonas stutzeri showed highest MIC value for lead up to 1300 mg/l concentration. The uptake of heavy metals, present in water and detoxification of metal ions by bacteria provide an additional mechanism of environmental bioremediation. The identified lead resistant bacteria could be useful for the bioremediation of lead contaminated sewage and waste water from various industries.

Keywords : Pseudomonas stutzeri, lead resistant bacteria, Minimum Inhibitory concentration (MIC), bioremediation, 16S rDNA sequencing.