Journal of Pure and Applied MicrobiologyVol. 11 No. 2

Isolation, Characterization and Antibiotic Sensitivity Pattern of Different Bacteria in Pus Sample

Swagata Roy and Debadatta Dhar (Chanda)

Department of Microbiology, Silchar Medical College & Hospital, Silchar, Assam 788014, India.

Received on 17 March 2017 and accepted on 01 May 2017



This study was undertaken to determine the frequently isolated organism from pus culture and to determine the antibiotic sensitivity patterns so that it helps to manage the drug resistant organism well in time which poses challenge to clinician to treat the patients. Total 2050 samples were collected from April 2016 to March 2017 to study antibiograms of various organisms. Organisms were identified as per standerd operative procedure and drug sensitivity was done as per CLSI guidelines. Most commonly encountered organism was Staph.aureus followed by Klebsiella, Pseudomonas, Proteus, Citrobacter, E.coli and Acinetobacter. Staph.aureus was showing sensitivity towards Vancomycin, whereas gram negative organisms were showing sensitivity towards Imipenem, Gentamycin, Ciprofloxacin and Ceftriaxone. Antimicrobial resistence is a predictable outcome of antimicrobial use. Lengthy or inappropriate antimicrobial therapy allow microbes to mutate into new forms that help them to survive antibiotic treatment and quickly become resistance strain. Knowledge of local common pathogens and their resistance status can guide clinician to choose appropriate antibiotic for empirical treatment of patients.

Keywords : Antimicrobial susceptibility, Pus, Pyogenic infection,Retrospective study.