Journal of Pure and Applied MicrobiologyVol. 11 No. 2

Comparing Culture and Multiplex PCR Methods to Examine Fastidious Bacteria in Otitis Externa and Media

Tina Delsouz Bahri1, Mehdi Goudarzi2, Seyed Mohammad Ghafoori3, Nasrin Ebrahimi4, Arezoo Asadi5 and Hossein Goudarzi6*

1Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3Department of Biotechnology, Payame Noor University, Tehran, Iran. 4Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 5Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 6Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Received on 15 February 2017 and accepted on 28 April 2017

 

ABSTRACT

Ear infection or otitis is a generic term used to refer to ear infection or inflammation. The purpose of the present study was to compare culture and Multiplex PCR methods to identify Alloiococcus otitidis, Moraxella catarrhalis, and Haemophilus influenza as three fastidious pathogens causing otitis. 100 patients, who had the physician-confirmed infection of the external ear and otitis media, were randomly sampled. The samples were analyzed with culture and Multiplex PCR. None of the three bacteria were found in samples of otitis externa by culture or PCR. In the case of otitis media three isolates of Alloiococcus otitidis, one isolation of Moraxella catarrhalis, and three strains of Haemophilus influenza were found using culture method. In the case of otitis media three isolates of Alloiococcus otitidis, one isolation of Moraxella catarrhalis, and three strains of Haemophilus influenza were found using culture method, but the rate of these bacteria were 25, 11 and 28 isolates using Multiplex PCR. The Multiplex PCR method was found to be furthermost sensitive, truthful and reproducible screening method for detection of fastidious bacteria.

Keywords : Otitis media, Otitis externa, Multiplex PCR, Fastidious bacteria.