Journal of Pure and Applied MicrobiologyVol. 11 No. 2

Optimization of Phospholipase A1 (PLA1) Production from a Soil Isolate Bacillus subtilis subsp. inaquosorum RG1 via Solid State Fermentation

Gaganpreet Kaur*1, Sanjeev Kumar Soni2 and Rupinder Tewari1

1Department of Microbial Biotechnology, South Campus, New Hospital Building, Panjab University, Sector 25, Chandigarh, 160014, India. 2Department of Microbiology, South Campus, Basic Medical Science (Block I), Panjab University, Sector 25, Chandigarh, 160014, India.

Received on 20 February 2017 and accepted on 15 April 2017

 

ABSTRACT

Microbial sources for phospholipase A1 (PLA1) are economic and industry relevant for degumming of oils and also their product lyso-phospholipids have been widely used as emulsifying agent. Numerous PLAs1 have been reported, but still the few enzymes have got position in the commercial sector. Due to enormous demand of PLA1 in the industrial sector, the present study was carried out to optimize PLA1 production using cheaper agro-industrial waste like defatted rice bran. For this, defatted rice bran was used in solid state fermentation for the production of PLA1.One-factor at a time approach was used to obtain maximum production of 51.5 U/gm which is 2.15 folds more than un-optimized medium. The optimized medium components were (pH 7): defatted rice bran (5gm), glucose (1% w/v), peptone (1% w/v) and olive oil (0.5 % v/v) with moisture content of 1:1.5 and after 48h of incubation at 37C. This approach will provide the cleaner solution for degumming of oil as compare to the acid degumming and also help to reduce the stress of the environment by utilizing waste. This is the first report where in Bacillus subtilis subsp. inaquosorum was employed for PLA1 production via solid state fermentation.

Keywords : Submerged fermentation, Solid-State Fermentation, Optimization, Classical approach, PLA1, Bacillis subtilis subsp. inaquosorum.