Journal of Pure and Applied MicrobiologyVol. 11 No. 2

Anaerobic Biodegradation of Polyaromatic Hydrocarbons by a Sulfate Reducing Bacteria C1Fd Strain

Sandra L. Villarreal Morales1, Nagamani Balagurusamy4, Raúl Rodríguez Herrera1, Alejandro Zugasti Cruz2, Mayela Govea Salas3 and Jesús Morlett Chávez1,2*

1Laboratory of Molecular Biology, Department of Food Science and Technology, School of Chemistry, 2Laboratory of Molecular Biology and Genetic Engineering, School of Chemistry, School of Chemistry, University Autonomous of Coahuila, Blvd. V. Carranza and Jose Cardenas V. s/n Col. Republica Ote., 25280 Saltillo, Coahuila, Mexico. 4Laboratory of Bioremediation, School of Biological Sciences, University Autonomous of Coahuila, Carretera Torreón- Matamoros Km. 7.5, 27000 Torreon, Coahuila, Mexico.

Received on 20 December 2016 and accepted on 15 January 2017

 

ABSTRACT

Aromatic hydrocarbons contamination is widely prevalent in various parts of the world due to anthropogenic activities and leads to anaerobic conditions. As a result, most of its biodegradation is due to anaerobic microorganisms, and most specifically by anaerobic bacteria capable of using sulfates as final electron acceptor to degrade these compounds. Although there are reports on consortia of microorganisms that are involved in the anaerobic biodegradation of monoaromatic hydrocarbons (MAH) and polyaromatic hydrocarbons (PAH), only few reports are available using pure cultures. This paper describes an anaerobic, gram positive and spore forming bacterial strain (C1Fd), which was isolated and purified from aromatic compounds degrading consortium developed using bovine rumen fluid as inoculum. C1Fd was able to use MAH and PAH under anaerobic conditions and removed up 9.4 mM of MAH and 9.2 mM of PAH in less than 72 h. The strain was identified as Bacillus sp. and is phylogenetically related to the hydrocarbon degrading bacteria, Desulfotomaculum sp., isolated from a wastewater treatment plant.

Keywords : Benzene, Naphthalene, Phenanthrene, Toluene.