Journal of Pure and Applied MicrobiologyVol. 11 No. 2

Urease-Producing Halophilic Bacteria Isolated from Bahr Al-Milh Salt Lake, Karbala, Iraq

Maryam Hosseini1, Fateme Babaha1, Mushtaq Talib Shawi Al-Rubaye1, Javad Fakhari1 and Mastafa Heilo Jabber Al-Musawi2

1Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran. 2College of Pharmacy, University of Al-Mustansriyah, Baghdad, Iraq.

Received on 10 April 2017 and accepted on 03 June 2017

 

ABSTRACT

Salt lakes represent an extreme habitat for prokaryotes which tolerate significant concentration of NaCl. Many studies on screening and characterization of these microorganisms have been done, however, very little reported about urease producer halophilic bacteria. The present study aimed to isolate urease positive bacteria from Bahr Al-Milh Salt Lake, Karbala, Iraq. A total of 161 gram positive and 57 gram negative bacteria were found which one third of them showed the ability to produce urease. 16S rDNA sequencing analysis showed the isolated bacteria belong to genus Bacillus, Virgibacillus, Halobacillus and Staphylococcus. Further studies focusing on the enzyme structures and encoding genes are suggested.

Keywords : halophiles, urease, screening.