Journal of Pure and Applied MicrobiologyVol. 11 No. 2

The Application of Mediated Biosynthesized Green Silver Nanoparticles by Streptomyces griseorubens in Water Treatment

Moustafa Y. El-Naggar1*, Wegdan Ramadan2 and Ramy A. El-Hamamsy3

1Botany and Microbiology Department, Faculty of Science, Alexandria University, Moharram Bey 21511, Egypt. 2Physics Department, Faculty of Science, Alexandria University, Egypt. 3Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt.

Received on 17 February 2017 and accepted on 11 April 2017

 

ABSTRACT

In the present study, a soil actinomycete was isolated from near the river Nile shoreline, Egypt. The identification of this isolate as Streptomyces griseorubens was performed using 16s rDNA. The sequence has been deposited in the Gene Bank with the accession number LC066679. Factors affecting the biogenesis of AgNPs were optimized by applying the Plackett-Burman factorial design. The maximum silver nanoparticles (AgNPs) biosynthesis (2.76 OD at 400 nm) was achieved in the trial No.9 that contained the following ingredients (g/L): Starch (20); MgSO (0.05); K HPO (1.0); NaNO (2.0); AgNO (1.0) mmol/l; PH (7); incubated at temperature 30C for 72 hr. The biosynthesized nanoparticles were characterized using spectroscopic techniques. AgNPs showed the characteristic UV spectra at a wavelength range 300 - 600 nm and a characteristic absorption peak was recorded at the wavelength of 400 nm. For AgNPs with absorbance height ofH2.56 a.u. and peak width at half maximum absorbance (PWHM) H120 nm which remained constant over a long period of time indicating its stability. FTIR spectra showed the functional group of the biomaterials capping the AgNPs. EDX confirmed the formation of the metallic silver nanoparticles, indicating the presence of proteinaceous cabbing. TEM micrograph showed spherical AgNPs in shape with an average diameter of 22 nm. The biosynthesized AgNPs showed high stability up-to two weeks. The conjugate (AgNPs/cellulosic fiber (C)) of Luffa aeygptiaca and (AgNPs/activated carbon (AC)) was applied for drinking water treatment, which resulted in fecal Coliform bacteria removal with a success of 99.9% as a water treatment application.

Keywords : Streptomyces griseorubens, AgNPs biosynthesis, AgNPs/cellulosic fiber, drinking water treatment application.