Journal of Pure and Applied MicrobiologyVol. 11 No. 1

Enhanced Adhesion and Cell Damage by Escherichia coli Harboring hly, papC and cnf-1genes to the Uroepithelium in Diabetic Mouse Bladder Model

H. Anandkumar1*, C.S. Vinodkumar3, G. Soham2, B. Basavaraj4, Achut Rao1 and H. Srinivasa2

1Department of Microbiology, Navodaya Medical College, Raichur, Karnataka, India. 2Department of Microbiology, St. Johnís Medical College, Bangalore, Karnataka, India. 3Department of Microbiology, SS Institute of Medical Sciences, Davangere, Karnataka, India. 4Department of Pathology, Navodaya Medical College, Raichur, Karnataka, India.

Received on 24 January 2017 and accepted on 04 March 2017

 

ABSTRACT

The in-vivo mouse bladder model was sought to determine the effect of virulent uropathogenic E. coli (UPEC) strain harboring hly, papC and cnf-1 genes on uroepithelium of diabetic mouse bladder infected transurethrally. The female BALB/c mice aged between 6-8 weeks were used in the study. The diabetes was induced by subcutaneous injection of alloxan hydrate (80mg / kg body weight) in mice. Two UPEC strains, one with hly, papC and cnf-1 virulent genes and the other (hypovirulent) without hly, cnf-1 and papC genes were selected for the study. The animals were anesthetized and 50 Ķl of bacterial inoculum was instilled in to bladder of DM and non-DM mice using specially devised mice catheter. The mice were sacrificed at 4 hrs, 24 hrs and 48 hrs of post infection, and the bladder was removed aseptically. One half of the bladder was homogenized and bacterial culture was performed. The other half of the bladder was used to document bacterial adhesion and invasion by histopathology and scanning electron microscopy. The exaggerated consequence of virulent UPEC strain on diabetic mouse bladder model was documented as enhanced adhesion and extensive damage of the uroepithelium of the bladder. However, hypovirulent UPEC strain failed to produce observable pathophysiological effect. Many of the UPEC were in the filamentous form and occasionally seen looping within and between adjacent superficial cells to escape from immune mechanism like micturation and exfoliation.

Keywords : UPEC; Escherichia coli; Mouse UTI model; Diabetes mellitus.