Journal of Pure and Applied MicrobiologyVol. 11 No. 1

Plant Growth Promoting Rhizobacteria of Curcuma amada (Mango ginger)

Ajay Kumar, Hariom Verma, Akhilesh Yadav, Waquar Akhtar Ansari, Prem Pratap Singh, Sandeep Kumar Singh, P.K. Singh and K.D. Pandey*

Centre of Advance study in Botany, Banaras Hindu University, Varanasi - 221 005, India.

Received on 15 August 2016 and accepted on 30 September 2016

 

ABSTRACT

In this study, 31 bacterial strains were isolated from the rhizospheric soil of Curcuma amada (mango ginger) and their plant growth promotion potential, salinity tolerance, antibiotic sensitivity, antimicrobial properties were evaluated. Eight bacterial strains namely Azotobacter chroococcum KCA1, Pseudomonas fluorescens KCA2, Bacillus subtilis KCA3, Bacillus sp. KCA4, Agrobacterium tumifaciens KCA5, Bacillus cereus KCL7, Pseudomonas putida KCA8 and Paenibacillus sp. KCA9 have been identified on the basis of biochemicals and 16S r RNA gene sequence analysis. All the strains solubilized tri-calcium phosphate and produced IAA, ammonia but only 50% of the strains produced siderophores during PGP traits analysis. Strains KCA8 tolerated maximum NaCl (7%) relative to strain KCA5 (1-2%). The strains were sensitive to the antibiotic chloromphenicol followed by erythromycin and most of these effectively inhibited growth of Escherichia coli, Fusarium solani and Alterneria alternata during antimicrobial properties.

Keywords : Curcuma amada, PGPR, PGP traits, Antimicrobial properties, Salinity tolerance.