Journal of Pure and Applied MicrobiologyVol. 11 No. 1

Effect of Nutrient Combinations of Cheaper Organic Substrates on the Mass Multiplication of Virulent Isolates of Trichoderma spp

U.R. Sangle1, Shivakumar S. Chinchure2, D.T. Meshram2 and K. Dhinesh Babu2

1ICAR Research Complex for Eastern Region, Patna-800 014 Bihar, India. 2ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra, India.

Received on 06 November 2016 and accepted on 13 February 2017

 

ABSTRACT

Ten substrates viz., Rice husk alone 100%, Rice husk 50% + Pigeonpea husk 50%, Rice husk 50% +Sorghum grain 50%, Rice husk 50% + Cow dung 50%, Pigeonpea husk alone 100%, Pigeonpea husk 50% + Sorghum grain 50%, Pigeonpea husk 50% + Cow dung 50%, Sorghum grain alone 100%, Sorghum grain 50% + Cow dung 50% and Cow dung 100% were taken up for mass multiplication of five isolates (2 of T. harzianum, 2 of T. viride and 1 of T. virens) were selected for study to find out the best mycelium growth and conidial production. In T18 (T. harzianum) the best sporulation was recorded in Rice husk 50% + Sorghum grain 50% after 21 days of incubation. In T25 (T. harzianum), the best sporulation was found in sorghum grain only even after 28 days of incubation. In T26 (T. viride), best sporulation was observed in rice husk alone at 100%. In T30 (T. virens), the best sporulation was found in Rice husk alone (100%).

Keywords : Biocontrol agent; mass multiplication; Organic substrates; Days; Trichoderma spp.