Journal of Pure and Applied MicrobiologyVol. 11 No. 1

Avian Paramyxovirus Serotype-1 Detection from Chicken Reared in Kashmir Valley

Rafia Maqbool1*, Shakil A. Wani1, Asifa Wali2, Zahid A. Kashoo1, Parvaiz S. Dar1, M. Younis Ganaie1, Sabia Qureshi1, Ishfaq Hussain1, Salik Nazki1, Aazima Shah3 and Najimaana Wani4

1Division of Veterinary Microbiology & Immunology, Faculty of Veterinary Science & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUASTK), India. 2Faculty of Fisheries, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUASTK), India. 3Division of Veterinary Pathology, Faculty of Veterinary Science & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology Of Kashmir (SKUASTK), India. 4Division of Veterinary Public Health, Faculty of Veterinary Science & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology Of Kashmir (SKUASTK), India.

Received on 23 September 2016 and accepted on 06 November 2016

 

ABSTRACT

Avian paramyxovirus serotype-1 (AMPV1) infects a wide range of avian species leading to broader range of clinical symptoms. The ease of transmission has allowed the virus to spread worldwide with varying degree of virulence depending upon virus strain and host range. Rapid detection is an important step to prevent an outbreak of the disease. The present study was carried out to detect APMV-1 from chicken reared in Kashmir Valley. Out of 12 suspected disease outbreaks, all were positive for AMPV-1. APMV-1 was detected using Matrix Protein gene (M gene) by RT-PCR. Detection by M gene is used for primary screening of the APMV-1 in chicken with both virulent and avirulent forms.

Keywords : Avian paramyxovirus serotype 1, Matrix protein gene, RT-PCR.