Journal of Pure and Applied MicrobiologyVol. 11 No. 1

Protective Efficiency of Pasteurella multocida A:1 Bacteriophage lysate Vaccine against Homologous and Heterologous Challenge in Poultry

Anshuja Nagdive1, Mayank Rawat1*, Bablu Kumar2, Ashok Kumar Tiwari1 and Somanath Chavhan3

1Division of Biological Standardization, Indian Veterinary Research Institute, Bareilly - 243 122, India. 2Division of Biological Products, Indian Veterinary Research Institute, Bareilly - 243 122, India. 3VD-1, Anturli, Tal-Muktainagar, Jalgoan-425306, India.

Received on 18 November 2016 and accepted on 04 January 2017

 

ABSTRACT

Fowl cholera (FC) caused by serotypes of Pasteurella multocida includes A:1, A:3, A:4 is a highly fatal septicemic disease. Preliminary trials of P. multocida A:1 bacteriophage lysate vaccine against FC was evaluated. Lytic phage and P. multocida ratio was standardized to obtain stable lysate batches. Consequently, three batches of lytic phage preparation were produced; estimation of protein and carbohydrate content amongst batches did not shown any significant variation indicating same batches can be produced by standardized procedure. Protective response trials in poultry with P. multocida A:1, A:3, A:4 against plain lysate and alum adsorbed lysate (1% alum) on vaccinated group showed both homologous and heterologous protection compared with inactivated whole cell group provided only homologous protection. Assessment of antibody response towards P. multocida A:1, A:3, A:4 antigen evaluated by Indirect Haemagglutination test (IHA) reveals presence of protective antibody titer.

Keywords : P. multocida, Bacteriophage lysate, Fowl cholera, Immune response, Indirect Haemagglutination test (IHA).