Journal of Pure and Applied MicrobiologyVol. 11 No. 1

Characterization of Probiotic Strains of Lactobacillus Candidates for Development of Synbiotic Product for Kazakh Population

Zinigul Sarmurzina1, Gulmira Bissenova2, Kunsulu Zakarya3, Raikhan Dospaeva4, Serik Shaikhin5 and Akhan Abzhalelov6

1Head of Laboratory of Microbiology in RSE "Republican Collection of Microorganisms" SC MES RK (Astana, Kazakhstan). 2Senior Researcher of Laboratory of Microbiology in RSE "Republican Collection of Microorganisms" SC MES RK (Astana, Kazakhstan). 3Deputy General Director for Science in RSE "Republican Collection of Microorganisms" SC MES RK (Astana, Kazakhstan). 4Junior researcher of Laboratory of Microbiology in RSE "Republican Collection of Microorganisms" SC MES RK (Astana, Kazakhstan). 5Head of Laboratory of Genetics and Biochemistry of Microorganisms in RSE "Republican Collection of Microorganisms" SC MES RK, (Astana, Kazakhstan). 6Director General in RSE "Republican Collection of Microorganisms " SC MES RK (Astana, Kazakhstan).

Received on 03 December 2016 and accepted on 20 January 2017

 

ABSTRACT

The health status of the individuals is determined by the microflora inhabiting the gastrointestinal tract. One way to restore the balance of the gastrointestinal microflora is the use of probiotics on the Lactobacillus strains. Probiotic bacteria must have a set of properties that allow them to compete with pathogens and opportunistic pathogens in the gut. Antimicrobial activity, antibiotic susceptibility, tolerance to bile and salt of Lactobacillus strains (Lactobacillus casei 3 B-RKM 0008, Lactobacillus plantarum 8RA-3 pl B-RKM 0015, Lactobacillus sakei 24a B-RKM 0559) we studied. The results showed that L. casei 3 B-RKM 0008 and L. plantarum 8RA-3 pl B-RKM 0015 showed antagonism toward conditionally pathogenic microorganisms, including Escherichia coli ATCC 25922 B-RKM 447, Staphylococcus aureus 209P B-RKM 0057, Serratia marcescens 221F B-RKM 0059, and Candida albicans ATCC 885-653 Y-RKM 0475. Strains of Lactobacillus were resistant to gentamicin, ofloxacin, ciprofloxacin, levofloxacin, cefotaxime, ceftriaxone, ceftazidime, amicacin. Lactobacillus strains to be able to grow in the bile (0.5-20%) and salt (2-6%). These results suggest that these strains may be used in the future as probiotic cultures for manufacturing of novel synbiotic product

Keywords : Lactobacillus, Antimicrobial activity, Antibiotic, Bile, Salt.