Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Production of Polyhydroxyalkanoates by Sugar Cane Rhizospheric Soil Bacterial Isolates

S. Dash, S. Mohapatra*, D.P. Samantaray and A.K. Sethi

Department of Microbiology, OUAT, Bhubaneswar-3, India.

Received on 21 July 2014 and accepted on 14 September 2014

 

ABSTRACT

In this research work, eight bacterial isolates were obtained from the rhizospheric soil region of sugarcane. Then the isolates were screened for the presence of PHA granule by following Sudan black staining of which, four bacterial isolates showed positive to Sudan black staining. However, the bacterial isolates showing PHA granule were Gram positive rod. Biochemical characterization revealed that all the bacterial isolates belongs to genus Bacillus. A negative trend in cellulase and pectinase activity were observed in case of all the PHA producing bacterial isolates. The optimum temperature and pH of all the bacterial isolates were found be 7 and 370C respectively. Under optimized condition Bacillus licheniformis produces 53.01% of polyhydroxyalkanoates in MSM culture medium. The production of PHA was found to be increased along with the increase in the biomass. The FTIR analysis of the extracted PHA showed the distinct peaks corresponding to C=O groups and the spectroscopic analysis gave proper insight for the chemical structure of PHB by reflecting the monomeric units. Further studies are required for elucidation of structure of the monpmer and to reduce the cost of production.

Keywords : Rhizospheric, Sudan-black, Biochemical, Polyhydroxyalkanoates & MSM.