Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Plant Growth Characteristics of Bacteria Isolated from Rhizosphere Region of Santalum album

A. Pradhan, B. Baisakh1 and B.B. Mishra*

Department of Microbiology, College of Basic Science & Humanities, 1Department of Plant Breeding and Genetics, College of Agriculture Orissa University of Agriculture and Technology, Bhubaneswar, -751 003, Odisha, India.

Received on 03 September 2014 and accepted on 10 November 2014

 

ABSTRACT

Plant growth promoting rhizobacteria (PGPR) have gained increasing importance in recent past due to agricultural benefit. On account of that, an attempt was made in the present investigation to study plant growth promoting activities of bacteria isolated from rhizospheric region of Santalum album (Sandalwood ) plant. A total of ten bacteria were isolated from the soil sample and amongst them two prominent phosphate solubilizers were selected for biochemical characterization and further study on plant growth promoting activities. The most potential among the two was tried with different crop plants to determine effect on germination and plant growth under laboratory and field conditions. Percent germination of Vigna radiata (Mung bean) in germination paper is 30%, and 6% in tray with sterilized soil & 5% in Pot culture method increased significantly over respective controls. A significant increase in seedling vigor index. Root length and shoot length at P ? 0.05 significant level was also observed. Morphological and biochemical characterization suggests the organism belongs to the genera Bacillus. 16S rRNA gene sequence submitted to NCBI gene bank was assigned with the accession number JQ408711.

Keywords : PGPR, Santalum album, Siderophore, IAA, HCN, 16S rRNA gene sequencing.