Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Organic Acid Production as a Function of Phosphate Solubilization by Pseudomonas aeruginosa Strain An-G Isolated from Temperate Zone of Himachal Pradesh

Ranjna Sharma1, Sheetal Rana1, S.P. Singh2 and Mohinder Kaur3

1PhD (Microbiology) Scholar. 2Scientist C (National Agri-Food Biotechnology Institute, Mohali, Punjab, India. 3Department of Basic Sciences, College of Forestry, Dr. YS Parmar University of Horticulture & Forestry, Nauni, Solan-173230 Himachal Pradesh, India.

Received on 20 July 2014 and accepted on 09 September 2014

 

ABSTRACT

An efficient phosphate solubilizing Pseudomonas aeruginosa strain An-G isolated from apple rhizosphere of Himachal Pradesh, exhibited solubilization of tricalcium phosphate (TCP) in Pikovskaya?s broth (47?g/ml). A decline in the pH of the medium was observed after three days of incubation at 28?2oC from 7.0 - 4.02 during the solubilization of phosphate substrate (TCP). Among the different organic acids which were estimated through HPLC-MS/MS, succinic acid was produced in higher concentration (2.016?g/ml). Other major organic acids produced were citric acid, malic acid and malonic acid in concentration 0.162 ?g/ml, 0.20 ?g/ml and 0.39?g/ml respectively. The production of fumaric acid, tartaric acid, quinic acid, lactic acid and schimic acid were observed in small amount during the solubilization of tricalcium phosphate.

Keywords : Pseudomonas aeruginosa, Phosphate solubilizing bacteria, TCP and Organic acids