Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Detection of Single Nucleotide Polymorphism in Growth Hormone Gene of Salmo trutta caspius

Abolhasan Rezaei*

Department of Genetics-School of Basic Science, Tonekabon Branch, Islamic, Azad University, Tonekabon, Iran.

Received on 11 August 2014 and accepted on 21 October 2014

 

ABSTRACT

In this research, I studied on the single nucleotide polymorphism (SNPs) of Growth hormone gene (GH) in Salmo trutta caspius. GH gene in Salmo trutta caspius have been six exons and five introns the full length. Single nucleotide polymorphism (SNPs) of Growth hormone gene (GH) were compared between Salmo trutta caspius and Salmo salar by BLAST- NCBI-Network system. Following to BLAST program, were found three gaps in full length of GH gene, first gap originated to 960 bp (Salmo trutta caspius) and 1610 bp (Salmo salar), second gap originated to 2033 bp (Salmo trutta caspius) and 4206 bp (Salmo salar), third gap 1318 bp (Salmo trutta caspius) and 2637 bp (Salmo salar). The all of gaps were not math with together, but the similarity of nucleotides between gaps were high. In this study we found SNPs in full length of GH gene when compared between Salmo trutta caspius and Salmo salar by BLAST- NCBI-Network system. According my research were found 22 single nucleotide mutations in first gap and 12 single nucleotide mutations in second gap, however there were not any single nucleotide mutation in third gap. For generally similarity of GH gene between Salmo trutta caspius and Salmo salar is high (98%).

Keywords : Salmo trutta caspius, single nucleotide polymorphism, Growth hormone gene, Salmo salar.